PittsburghV1, Main, Exploration, bibRecord, 000798

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Identifieur interne : 000798 ( Main/Exploration ); précédent : 000797; suivant : 000799

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Auteurs : Kathrin Gassei [États-Unis] ; Hanna Valli ; Kyle E. Orwig

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2014.

RBID : pubmed:25173170

Descripteurs français

KwdFr :
- Animaux, Canalicules séminifères (cytologie), Canalicules séminifères (métabolisme), Cellules souches (cytologie), Cellules souches (métabolisme), Différenciation cellulaire, Haplorhini, Humains, Immunohistochimie (), Lignage cellulaire, Mâle, Souris, Spermatogenèse, Spermatogonies (cytologie), Spermatogonies (métabolisme).
MESH :
- cytologie : Canalicules séminifères, Cellules souches, Spermatogonies.
- métabolisme : Canalicules séminifères, Cellules souches, Spermatogonies.
- Animaux, Différenciation cellulaire, Haplorhini, Humains, Immunohistochimie, Lignage cellulaire, Mâle, Souris, Spermatogenèse.

English descriptors

KwdEn :
- Animals, Cell Differentiation, Cell Lineage, Haplorhini, Humans, Immunohistochemistry (methods), Male, Mice, Seminiferous Tubules (cytology), Seminiferous Tubules (metabolism), Spermatogenesis, Spermatogonia (cytology), Spermatogonia (metabolism), Stem Cells (cytology), Stem Cells (metabolism).
MESH :
- cytology : Seminiferous Tubules, Spermatogonia, Stem Cells.
- metabolism : Seminiferous Tubules, Spermatogonia, Stem Cells.
- methods : Immunohistochemistry.
- Animals, Cell Differentiation, Cell Lineage, Haplorhini, Humans, Male, Mice, Spermatogenesis.

Abstract

Spermatogonial stem cells (SSCs) and undifferentiated progenitor spermatogonia in mammalian seminiferous tubules are organized in chains, connected by intracellular bridges. Clone size is generally related to stem cell potential, with shorter chains containing the majority of the stem cell population. Immunofluorescence detection of spermatogonia-specific proteins in whole-mount seminiferous tubule preparations is the only method that allows researchers to relate clone size with the molecular phenotype in spermatogenic lineage development. Here we describe in detail the method used to detect nuclear, cytoplasmic, and cell surface molecules in seminiferous tubules isolated from mouse, monkey, and human testes.

DOI: 10.1007/978-1-4939-1435-7_15
PubMed: 25173170

Affiliations:

États-Unis

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.</title>
<author><name sortKey="Gassei, Kathrin" sort="Gassei, Kathrin" uniqKey="Gassei K" first="Kathrin" last="Gassei">Kathrin Gassei</name>
<affiliation wicri:level="1"><nlm:affiliation>Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213</wicri:regionArea>
<wicri:noRegion>15213</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Valli, Hanna" sort="Valli, Hanna" uniqKey="Valli H" first="Hanna" last="Valli">Hanna Valli</name>
</author>
<author><name sortKey="Orwig, Kyle E" sort="Orwig, Kyle E" uniqKey="Orwig K" first="Kyle E" last="Orwig">Kyle E. Orwig</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:25173170</idno>
<idno type="pmid">25173170</idno>
<idno type="doi">10.1007/978-1-4939-1435-7_15</idno>
<idno type="wicri:Area/PubMed/Corpus">003E50</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003E50</idno>
<idno type="wicri:Area/PubMed/Curation">003E23</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003E23</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003E23</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003E23</idno>
<idno type="wicri:Area/Ncbi/Merge">003259</idno>
<idno type="wicri:Area/Ncbi/Curation">003259</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003259</idno>
<idno type="wicri:Area/Main/Merge">000800</idno>
<idno type="wicri:Area/Main/Curation">000798</idno>
<idno type="wicri:Area/Main/Exploration">000798</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.</title>
<author><name sortKey="Gassei, Kathrin" sort="Gassei, Kathrin" uniqKey="Gassei K" first="Kathrin" last="Gassei">Kathrin Gassei</name>
<affiliation wicri:level="1"><nlm:affiliation>Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213</wicri:regionArea>
<wicri:noRegion>15213</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Valli, Hanna" sort="Valli, Hanna" uniqKey="Valli H" first="Hanna" last="Valli">Hanna Valli</name>
</author>
<author><name sortKey="Orwig, Kyle E" sort="Orwig, Kyle E" uniqKey="Orwig K" first="Kyle E" last="Orwig">Kyle E. Orwig</name>
</author>
</analytic>
<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
<idno type="eISSN">1940-6029</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Differentiation</term>
<term>Cell Lineage</term>
<term>Haplorhini</term>
<term>Humans</term>
<term>Immunohistochemistry (methods)</term>
<term>Male</term>
<term>Mice</term>
<term>Seminiferous Tubules (cytology)</term>
<term>Seminiferous Tubules (metabolism)</term>
<term>Spermatogenesis</term>
<term>Spermatogonia (cytology)</term>
<term>Spermatogonia (metabolism)</term>
<term>Stem Cells (cytology)</term>
<term>Stem Cells (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Canalicules séminifères (cytologie)</term>
<term>Canalicules séminifères (métabolisme)</term>
<term>Cellules souches (cytologie)</term>
<term>Cellules souches (métabolisme)</term>
<term>Différenciation cellulaire</term>
<term>Haplorhini</term>
<term>Humains</term>
<term>Immunohistochimie ()</term>
<term>Lignage cellulaire</term>
<term>Mâle</term>
<term>Souris</term>
<term>Spermatogenèse</term>
<term>Spermatogonies (cytologie)</term>
<term>Spermatogonies (métabolisme)</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Canalicules séminifères</term>
<term>Cellules souches</term>
<term>Spermatogonies</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Seminiferous Tubules</term>
<term>Spermatogonia</term>
<term>Stem Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Seminiferous Tubules</term>
<term>Spermatogonia</term>
<term>Stem Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunohistochemistry</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Canalicules séminifères</term>
<term>Cellules souches</term>
<term>Spermatogonies</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Differentiation</term>
<term>Cell Lineage</term>
<term>Haplorhini</term>
<term>Humans</term>
<term>Male</term>
<term>Mice</term>
<term>Spermatogenesis</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Différenciation cellulaire</term>
<term>Haplorhini</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Lignage cellulaire</term>
<term>Mâle</term>
<term>Souris</term>
<term>Spermatogenèse</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Spermatogonial stem cells (SSCs) and undifferentiated progenitor spermatogonia in mammalian seminiferous tubules are organized in chains, connected by intracellular bridges. Clone size is generally related to stem cell potential, with shorter chains containing the majority of the stem cell population. Immunofluorescence detection of spermatogonia-specific proteins in whole-mount seminiferous tubule preparations is the only method that allows researchers to relate clone size with the molecular phenotype in spermatogenic lineage development. Here we describe in detail the method used to detect nuclear, cytoplasmic, and cell surface molecules in seminiferous tubules isolated from mouse, monkey, and human testes.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Orwig, Kyle E" sort="Orwig, Kyle E" uniqKey="Orwig K" first="Kyle E" last="Orwig">Kyle E. Orwig</name>
<name sortKey="Valli, Hanna" sort="Valli, Hanna" uniqKey="Valli H" first="Hanna" last="Valli">Hanna Valli</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Gassei, Kathrin" sort="Gassei, Kathrin" uniqKey="Gassei K" first="Kathrin" last="Gassei">Kathrin Gassei</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Amérique/explor/PittsburghV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000798 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000798 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Amérique
   |area=    PittsburghV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:25173170
   |texte=   Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25173170" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PittsburghV1

This area was generated with Dilib version V0.6.38.
Data generation: Fri Jun 18 17:37:45 2021. Site generation: Fri Jun 18 18:15:47 2021

Serveur d'exploration sur Pittsburgh

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur Pittsburgh
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.